Journal: NPJ Breast Cancer
Article Title: Integrated profiling of metaplastic breast cancer identifies putative master regulators of intratumoral heterogeneity
doi: 10.1038/s41523-025-00807-x
Figure Lengend Snippet: A Protein expression for MAL2, SPP1, IBSP, and BGN was evaluated by IHC H-score (mean with SD). Asterisks indicate statistical significance: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. The x-axis indicates phenotype and sample size. B High-power image of the osteoid matrix in case two. C Transcriptomic changes for IBSP in the osteoid matrix, with arrows indicating expression levels from low to high. D IHC for IBSP shows protein expression changes in the osteoid matrix, with arrows indicating expression levels from low to high. B – D show the same region. E High-power image of the spindle to pleomorphic transition region in case one. F Transcriptomic changes for SPP1 from spindle to pleomorphic, with arrows indicating expression levels from low to high. G IHC for SPP1 shows expression changes from low (spindle) to high (pleomorphic). E – G show the same region.
Article Snippet: The slides were incubated with primary antibodies targeting MAL2 (Merck, ZRB2741, 1:100), SPP1 (Merck, HPA027541, 1:50), IBSP (Invitrogen, PA550633 , 1:200), and BGN (Abcam, EPR20235 , 1:5000) for 16 hours at 4°C.
Techniques: Expressing